|
| Status |
Public on Jun 30, 2007 |
| Title |
Ovary 3, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
mature ovary
|
| Organism |
Oryza sativa |
| Characteristics |
genotype: Japonica Nipponbare
age: ovaries at anthesis
age: rice ovary before pollination
|
| Growth protocol |
natural conditions,growing season begins from December to next April, in an experimental farm in Hainan, China.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
remove lemma and palea from floret, the stigma is extracted from pistil, the remainder of the pistil containing the style is used as the ovary samples.
total RNA was isolated and purified by Qiagen RNeasy kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from rice stigma
|
| Data processing |
The data were analyzed with GCOS software (Microarray Suite version 5.0, MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
May 29, 2007 |
| Last update date |
Jul 31, 2009 |
| Contact name |
Wenying Xu |
| E-mail(s) |
wyxu@genetics.ac.cn
|
| Organization name |
Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences
|
| Street address |
Datun Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE7951 |
Genome-wide gene expression profiling of rice stigma |
|
