|Public on Jun 30, 2007
|stigma 2, biological rep 2
|genotype: Japonica Nipponbare
age: unpollinated stigmas at anthesis
age: rice stigma before pollination
|natural conditions,growing season begins from December to next April, in an experimental farm in Hainan, China.
|remove lemma and palea from floret, the stigma is extracted by cutting the pistil just below the base of the plumose stigma
total RNA was isolated and purified by Qiagen RNeasy kit
|Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|Gene expression data from rice stigma
|The data were analyzed with GCOS software (Microarray Suite version 5.0, MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|May 29, 2007
|Last update date
|Jul 31, 2009
|Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences
|Datun Road, Chaoyang District
|Genome-wide gene expression profiling of rice stigma