|
| Status |
Public on May 01, 2016 |
| Title |
Skin_Fibroblast_c182 |
| Sample type |
genomic |
| |
|
| Source name |
Skin biopsy, fibroblast, cell 182
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
tissue: skin fibroblast
|
| Treatment protocol |
Following overnight treatment of the biopt with 400U/ml collagenase in culture medium, single cells were washed in 1xPBS (Thermo Scientific, USA).
|
| Growth protocol |
Single fibroblasts were isolated from a fresh skin biopsy.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The genome of collected single cells was amplified using GenomiphiV2 (GE Healthcare).
|
| Label |
Cy5,Cy3
|
| Label protocol |
The samples were not labelled before the hybridization. The staining is based on post-hybridization Cy5/Cy3 staining by extension, according to the manual of Infinium II Assay (Illumina, USA).
|
| |
|
| Hybridization protocol |
According to the manual of Infinium II Assay (Illumina, USA)
|
| Scan protocol |
According to the manual of Infinium II Assay (Illumina, USA)
|
| Description |
Genomiphi V2 whole genome amplified material.
|
| Data processing |
Raw data were further processed using GenomeStudio.
|
| |
|
| Submission date |
Nov 17, 2015 |
| Last update date |
May 01, 2016 |
| Contact name |
Eftychia Dimitriadou |
| E-mail(s) |
eftychia.dimitriadou@med.kuleuven.be
|
| Organization name |
KU Leuven
|
| Lab |
Cytogenetics and Genome Research
|
| Street address |
Herestraat 49, ON1, bus 602
|
| City |
Leuven |
| ZIP/Postal code |
3000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL13829 |
| Series (2) |
| GSE75098 |
Segmental chromosomal imbalances arise at high frequency in human fibroblasts (SB_sc_2) |
| GSE75116 |
Segmental chromosomal imbalances arise at high frequency in human fibroblasts |
|
