|
| Status |
Public on May 01, 2016 |
| Title |
Fibroblast_S2_c65_sc2 |
| Sample type |
genomic |
| |
|
| Source name |
Primary fibroblast cell line S2, cell 65, sister cell 2
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
cell line: S2
|
| Treatment protocol |
Single fibroblasts were plated separately and the two cells that derived following a cell division were isolated individually.
|
| Growth protocol |
Primary fibroblasts were grown in 75-cm2 plastic flasks (BD Falcon, USA) under standard culture conditions in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) (Gibco, USA) medium complemented with 10% Fetal Bovine Serum (FBS) (Thermo Scientific, USA).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The genome of collected single cells was amplified using either Genomiphi V2 (GE Healthcare, UK) or RepliG-sc (Qiagen, NL) whole genome amplification kit.
|
| Label |
Cy5,Cy3
|
| Label protocol |
The samples were not labelled before the hybridization. The staining is based on post-hybridization Cy5/Cy3 staining by extension, according to the manual of Infinium II Assay (Illumina, USA).
|
| |
|
| Hybridization protocol |
According to the manual of Infinium II Assay (Illumina, USA)
|
| Scan protocol |
According to the manual of Infinium II Assay (Illumina, USA)
|
| Description |
RepliG-sc whole genome amplified material.
|
| Data processing |
Raw data were further processed using GenomeStudio.
|
| |
|
| Submission date |
Nov 17, 2015 |
| Last update date |
May 01, 2016 |
| Contact name |
Eftychia Dimitriadou |
| E-mail(s) |
eftychia.dimitriadou@med.kuleuven.be
|
| Organization name |
KU Leuven
|
| Lab |
Cytogenetics and Genome Research
|
| Street address |
Herestraat 49, ON1, bus 602
|
| City |
Leuven |
| ZIP/Postal code |
3000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL13829 |
| Series (2) |
| GSE75088 |
Segmental chromosomal imbalances arise at high frequency in human fibroblasts (S2) |
| GSE75116 |
Segmental chromosomal imbalances arise at high frequency in human fibroblasts |
|
