|
| Status |
Public on Nov 03, 2015 |
| Title |
cultured PBMC from MS patient stimulated with PHA |
| Sample type |
RNA |
| |
|
| Source name |
cultured MS PBMC with PHA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: PBMC
disease state: MS patient
gender: female
age: 40
|
| Treatment protocol |
To induce PBMC activation, Phytohemagglutinin (PHA) (ThermoFisher) (0.5%) was used.
|
| Growth protocol |
PBMCs were isolated from whole blood and cryopreserved in liquid nitrogen until used. After thawing, PBMCs were cultured in a P48 plate (200.000cells/well) for 72h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled using FlashTag Biotin HSR RNA labeling kit (Genisphere)
|
| |
|
| Hybridization protocol |
GeneChip Hybridization, Wash and Stain kit was used for hybridization in a GeneChip Hybridization oven 640 and in a GeneChip Fluidics Station 450 using the protocol FS450-0002, as explained in the protocol provided by Affymetrix.
|
| Scan protocol |
Arrays were scanned in a GeneChip 7G scanner and analysed in Affymetrix GeneChip Command Console Scan Control v4.0.0.1567.
|
| Description |
03-029EM_PHA
|
| Data processing |
Quality control, normalization and summarization of the data were performed in Expression Console software build 1.4.0.38 (Affymetrix). Only human probes.
|
| |
|
| Submission date |
Nov 02, 2015 |
| Last update date |
Nov 03, 2015 |
| Contact name |
Maider Muñoz-Culla |
| E-mail(s) |
maider.munoz@ehu.eus
|
| Organization name |
Biodonostia Institute
|
| Street address |
Paseo Dr.Begiristain s/n
|
| City |
San Sebastian |
| ZIP/Postal code |
20014 |
| Country |
Spain |
| |
|
| Platform ID |
GPL19117 |
| Series (2) |
| GSE74582 |
Small nucleolar RNA and microRNA expression data from an MS patient's and a healthy control's PBMC cultures stimulated with phytohemagglutinin. |
| GSE74586 |
SncRNA (microRNA & snoRNA) expression in leucocytes from multiple sclerosis relapse and remission and cultured peripheral blood mononuclear cells |
|
