|
| Status |
Public on Aug 22, 2017 |
| Title |
ISX n3 |
| Sample type |
RNA |
| |
|
| Source name |
Long-term cultured Human Pancreatic Islet cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Pancreatic Islet cells, long-term culture
|
| Treatment protocol |
Long-term cultured human pancreatic islet cells were treated with ISX (20uM) or DMSO (0.1%) for 7d with drug/media replacement at day 4.
|
| Growth protocol |
Human pacreatic islets were cultured for 8-14 months in RPMI-1640 with 10% FBS and Pen/Strep prior to treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the PureLink RNA Mini Kit (Life Technologies) per manufacturers instructions. On-column DNAse treatment was performed during the purification to eliminate trace genomic DNA.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT Plus cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human HTA Arrays (Affymetrix)
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
CHP file generated for Alternative Splicing Analysis from the Expression Console software and then analyzed in the Transcriptome Analysis Console v2.0 for log2 expression values.
|
| Data processing |
Raw data were processed with the Expression Console (Affymetrix) for RMA summarization and Quantile normalization.
|
| |
|
| Submission date |
Oct 21, 2015 |
| Last update date |
Aug 22, 2017 |
| Contact name |
Michael A Kalwat |
| E-mail(s) |
mkalwat@indianabiosciences.org, mkalwat@iu.edu
|
| Organization name |
Indiana Biosciences Research Institute
|
| Department |
Lilly Diabetes Center of Excellence
|
| Lab |
Kalwat Lab
|
| Street address |
1210 Waterway Blvd Suite 2000
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46077 |
| Country |
USA |
| |
|
| Platform ID |
GPL17585 |
| Series (1) |
| GSE74240 |
Effects of isoxazole (ISX) on long-term cultured human islets |
|
