Male C57Bl/6 (retired breeders 25-35 weeks old) purchased from the Jackson Laboratory. Abdominal (epidydimal) adipose tissue was cut into small pieces and digested for about 40 minutes with collagease type II. Cell suspensions were then filtered through a sieve and stromovascular fraction (SVF) was harvested after spinning. Genetic background: Male C57Bl/6; Age: 25-35 weeks old; Number of mice per chip: 8-10; Tissue: Abdominal (epidydimal) adipose tissue; Sorting: B220-, CD11b-, CD8a-, CD4+, TCRb+, CD25+
Extracted molecule
total RNA
Extraction protocol
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label
Biotin
Label protocol
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
Hybridization protocol
Per Joslin Diabetes Center microarray core facility.
Scan protocol
Per Joslin Diabetes Center microarray core facility.
