|
| Status |
Public on Oct 01, 2015 |
| Title |
CSC+Exo 2 |
| Sample type |
RNA |
| |
|
| Source name |
CSCs treated with MSC-exosomes from bone marrow
|
| Organism |
Rattus norvegicus |
| Characteristics |
background strain: Sprague Dawley
tissue: heart
cell type: cardiac stem cells (CSCs)
age: 4 weeks
|
| Treatment protocol |
For miRNA array analysis, CSCs were grown in culture medium supplemented with MSC-exosomes for 3 consecutive days. Then cells were collected for RNA extraction and subsequent experiments.
|
| Growth protocol |
Cardiac stem cells were isolated and cultured as previously reported. MSCs were isolated and cultured until Passage 4. Then exosomes from MSCs were extracted using Exo-Quick-TCTM (System Bioscience) according to the user manual.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
Per RNA sample, 1 ug were used as input into the procedure as recommended by protocol (Genisphere FlashTagTM Biotin RNA Labeling for Affymetrix GeneChip miRNA arrays). 1ug of total RNA was used as input into the labeling reaction.
|
| |
|
| Hybridization protocol |
Labeled miRNA was hybridized to the array for 16 hours at 48 “C and 60 rpm as described in the protocol.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G
|
| Description |
miRNA expression profiling from CSCs treated with MSC-exosomes
|
| Data processing |
Expression data were generated by Affymetrix Expression Console software and normalized by the MAS5 method.
|
| |
|
| Submission date |
Sep 30, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Junjie Yang |
| E-mail(s) |
jjyang@suda.edu.cn
|
| Phone |
0086-532-67780948
|
| Organization name |
Institute for cardiovascular science of Soochow University
|
| Street address |
708 Renmin Road
|
| City |
Suzhou |
| ZIP/Postal code |
215006 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE73598 |
Expression data from cardiac stem cells (CSCs) with or without MSC-exosomes treatment |
|
