|
| Status |
Public on Sep 28, 2018 |
| Title |
muscle_D65_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
mouse, skeletal muscle, postnatal 65 days,replicate 4
|
| Organism |
Mus musculus |
| Characteristics |
tissue: skeletal muscle
ages: postnatal 65 days
strain: ICR
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the TRIzol Reagent (Invitrogen, Carlsbad, CA, USA). Genomic DNA was removed by DNase I (Qiagen, Beijing, China).mRNA was purified from total RNA after removal of rRNA. Integrity and concentration of RNA was assessed by the NanoDrop ND-1000.
|
| Label |
Cy3
|
| Label protocol |
The sample preparation and microarray hybridization were performed based on the manufacturer’s standard protocols with minor modifications. Briefly, mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then, each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3’ bias utilizing a random priming method.
|
| |
|
| Hybridization protocol |
The labeled cRNAs were hybridized onto the Mouse LncRNA Array v2.0 (8 x 60K, Arraystar).
|
| Scan protocol |
slides were scanned with the Agilent DNA Microarray Scanner. Data was extracted using Agilent Feature Extraction software. Normalization was performed using Agilent GeneSpring GX 11.5.1.
|
| Data processing |
Data analysis was performed using Agilent GeneSpring GX 11.5.1 software.
|
| |
|
| Submission date |
Sep 29, 2015 |
| Last update date |
Sep 28, 2018 |
| Contact name |
Zhonglin Tang |
| E-mail(s) |
zhonglinqy_99@sina.com
|
| Phone |
+86-10-62813822
|
| Organization name |
Chinese Academy of Agricultural Sciences
|
| Department |
Institute of Animal Science
|
| Lab |
Department of Gene and Cell Engineering
|
| Street address |
No. 2 Yuanmingyuan West Road, Beijing, 100193, P.R.China,
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL15691 |
| Series (1) |
| GSE73575 |
LncRNAs expression profiling of mouse skeletal muscle at postnatal 0 and 65 days |
|
