|
| Status |
Public on Feb 17, 2017 |
| Title |
HFL-1 siNTC, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
siNTC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HFL-1
transfection: siNTC
|
| Treatment protocol |
Cells were transfected with siRNA, using Lipofectamine RNAiMAX (Invitrogen), according to the manufacturer’s instructions.Total RNA was extracted from HFL-1 cells 48h after transfection.
|
| Growth protocol |
Standard HFL-1 growth condition.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (Qiagen) was used for total RNA extraction.
RNA libraries were prepared for sequencing using standard Ion Torrent protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent Proton |
| |
|
| Data processing |
BAM Alignment to Hg19 (TMAP version 3.4.1, the Torrent Mapping Alignment Program for Ion Torrent Data)
Read count analysis (HTseq version 0.6.1, Ensemble annotation​)
Genome_build: Hg19
Supplementary_files_format_and_content: tab-delimited text file include raw read count values for each Sample
|
| |
|
| Submission date |
Sep 29, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Satoshi Noguchi |
| E-mail(s) |
snoguchi-tky@umin.ac.jp
|
| Organization name |
Graduate School of Medicine, The University of Tokyo
|
| Department |
Department of Respiratory Medicine
|
| Street address |
7-3-1 Hongo, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17303 |
| Series (1) |
| GSE73555 |
Genes regulated by TAZ in a lung fibroblast cell line |
|
| Relations |
| BioSample |
SAMN04121996 |
| SRA |
SRX1295478 |
