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Sample GSM18956 Query DataSets for GSM18956
Status Public on Mar 19, 2004
Title 3AJZ02022760_kidney
Sample type RNA
 
Source name 3AJZ02022760_kidney
Organism Homo sapiens
Characteristics 3AJZ02022760_kidney
Extracted molecule total RNA
Extraction protocol Human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA), Clontech, AllCells (Berkeley, CA), Clonetics/BioWhittaker (Walk-ersville, MD), AMS Biotechnology (Abingdon, Oxfordshire, U.K.), and the University of California at San Diego. When samples from four or more subjects were available, equal numbers of male and female subjects were used to make two independent pools; when fewer than four samples were available, RNA samples were pooled, and duplicate amplifications were performed for each pool (detailed annotation for human samples is on our web site, http:\\symatlas.gnf.org) Extracted RNA was additionally purified on RNeasy mini columns (Qiagen). RNA quantity and quality was verified on Bioanalyser 2100 (Agilent).
Label biotin
Label protocol Five micrograms of total RNA was used to synthesize cDNA that was then used as a template to generate biotinylated cRNA using Enzo kits.
 
Hybridization protocol standard Affymetrix procedures except for use of a custom 24-channel fluidics machine and GCS2500 scanner
Scan protocol standard Affymetrix procedures
Description kidney
Data processing MAS5
 
Submission date Mar 18, 2004
Last update date Sep 25, 2006
Contact name John R Walker
E-mail jwalker@gnf.org
Phone 858-812-1636
Organization name Genomics Institute of the Novartis Research Foundation
Lab Genetics Core
Street address 10675 John Jay Hopkins
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platform ID GPL96
Series (1)
GSE1133 tissue-specific pattern of mRNA expression

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript

Data table
ID_REF VALUE
AFFX-BioB-5_at 619.4
AFFX-BioB-M_at 996.7
AFFX-BioB-3_at 507.6
AFFX-BioC-5_at 1748.2
AFFX-BioC-3_at 1518.9
AFFX-BioDn-5_at 1793.1
AFFX-BioDn-3_at 12828.5
AFFX-CreX-5_at 13381.2
AFFX-CreX-3_at 21194.8
AFFX-DapX-5_at 6
AFFX-DapX-M_at 71
AFFX-DapX-3_at 10.2
AFFX-LysX-5_at 53.1
AFFX-LysX-M_at 18.5
AFFX-LysX-3_at 52.8
AFFX-PheX-5_at 3.6
AFFX-PheX-M_at 18
AFFX-PheX-3_at 24.2
AFFX-ThrX-5_at 68.4
AFFX-ThrX-M_at 20.4

Total number of rows: 22283

Table truncated, full table size 356 Kbytes.




Supplementary file Size Download File type/resource
GSM18956.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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