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Sample GSM18955 Query DataSets for GSM18955
Status Public on Mar 19, 2004
Title 3AJZ02022643_kidney
Sample type RNA
 
Source name 3AJZ02022643_kidney
Organism Homo sapiens
Characteristics 3AJZ02022643_kidney
Extracted molecule total RNA
Extraction protocol Human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA), Clontech, AllCells (Berkeley, CA), Clonetics/BioWhittaker (Walk-ersville, MD), AMS Biotechnology (Abingdon, Oxfordshire, U.K.), and the University of California at San Diego. When samples from four or more subjects were available, equal numbers of male and female subjects were used to make two independent pools; when fewer than four samples were available, RNA samples were pooled, and duplicate amplifications were performed for each pool (detailed annotation for human samples is on our web site, http:\\symatlas.gnf.org) Extracted RNA was additionally purified on RNeasy mini columns (Qiagen). RNA quantity and quality was verified on Bioanalyser 2100 (Agilent).
Label biotin
Label protocol Five micrograms of total RNA was used to synthesize cDNA that was then used as a template to generate biotinylated cRNA using Enzo kits.
 
Hybridization protocol standard Affymetrix procedures except for use of a custom 24-channel fluidics machine and GCS2500 scanner
Scan protocol standard Affymetrix procedures
Description kidney
Data processing MAS5
 
Submission date Mar 18, 2004
Last update date Sep 25, 2006
Contact name John R Walker
E-mail jwalker@gnf.org
Phone 858-812-1636
Organization name Genomics Institute of the Novartis Research Foundation
Lab Genetics Core
Street address 10675 John Jay Hopkins
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platform ID GPL96
Series (1)
GSE1133 tissue-specific pattern of mRNA expression

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript

Data table
ID_REF VALUE
AFFX-BioB-5_at 700.5
AFFX-BioB-M_at 1104.9
AFFX-BioB-3_at 601.7
AFFX-BioC-5_at 2059.7
AFFX-BioC-3_at 1913.9
AFFX-BioDn-5_at 1858.2
AFFX-BioDn-3_at 14413.6
AFFX-CreX-5_at 17065.8
AFFX-CreX-3_at 26137.4
AFFX-DapX-5_at 59
AFFX-DapX-M_at 98.1
AFFX-DapX-3_at 23.4
AFFX-LysX-5_at 78
AFFX-LysX-M_at 105.8
AFFX-LysX-3_at 32.7
AFFX-PheX-5_at 10.6
AFFX-PheX-M_at 106.3
AFFX-PheX-3_at 53.7
AFFX-ThrX-5_at 35.6
AFFX-ThrX-M_at 164.8

Total number of rows: 22283

Table truncated, full table size 359 Kbytes.




Supplementary file Size Download File type/resource
GSM18955.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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