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Sample GSM18936 Query DataSets for GSM18936
Status Public on Mar 19, 2004
Title 3AJZ02040819_brainThalamus
Sample type RNA
 
Source name 3AJZ02040819_brainThalamus
Organism Homo sapiens
Characteristics 3AJZ02040819_brainThalamus
Extracted molecule total RNA
Extraction protocol Human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA), Clontech, AllCells (Berkeley, CA), Clonetics/BioWhittaker (Walk-ersville, MD), AMS Biotechnology (Abingdon, Oxfordshire, U.K.), and the University of California at San Diego. When samples from four or more subjects were available, equal numbers of male and female subjects were used to make two independent pools; when fewer than four samples were available, RNA samples were pooled, and duplicate amplifications were performed for each pool (detailed annotation for human samples is on our web site, http:\\symatlas.gnf.org) Extracted RNA was additionally purified on RNeasy mini columns (Qiagen). RNA quantity and quality was verified on Bioanalyser 2100 (Agilent).
Label biotin
Label protocol Five micrograms of total RNA was used to synthesize cDNA that was then used as a template to generate biotinylated cRNA using Enzo kits.
 
Hybridization protocol standard Affymetrix procedures except for use of a custom 24-channel fluidics machine and GCS2500 scanner
Scan protocol standard Affymetrix procedures
Description brainThalamus
Data processing MAS5
 
Submission date Mar 18, 2004
Last update date Sep 25, 2006
Contact name John R Walker
E-mail(s) jwalker@gnf.org
Phone 858-812-1636
Organization name Genomics Institute of the Novartis Research Foundation
Lab Genetics Core
Street address 10675 John Jay Hopkins
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platform ID GPL96
Series (1)
GSE1133 tissue-specific pattern of mRNA expression

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript

Data table
ID_REF VALUE
AFFX-BioB-5_at 1493.4
AFFX-BioB-M_at 3383.6
AFFX-BioB-3_at 1942.2
AFFX-BioC-5_at 5562.2
AFFX-BioC-3_at 4506.6
AFFX-BioDn-5_at 5935.3
AFFX-BioDn-3_at 23634.6
AFFX-CreX-5_at 45617.6
AFFX-CreX-3_at 57241
AFFX-DapX-5_at 18.1
AFFX-DapX-M_at 131.9
AFFX-DapX-3_at 12.1
AFFX-LysX-5_at 85.3
AFFX-LysX-M_at 23.3
AFFX-LysX-3_at 3.6
AFFX-PheX-5_at 9.9
AFFX-PheX-M_at 13.3
AFFX-PheX-3_at 96.2
AFFX-ThrX-5_at 22.6
AFFX-ThrX-M_at 19.7

Total number of rows: 22283

Table truncated, full table size 357 Kbytes.




Supplementary file Size Download File type/resource
GSM18936.CEL.gz 3.3 Mb (ftp)(http) CEL

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