|
| Status |
Public on Sep 06, 2016 |
| Title |
Liver_DW_4hr_no4 |
| Sample type |
RNA |
| |
|
| Source name |
Liver of mouse administrated water
|
| Organism |
Mus musculus |
| Characteristics |
strain: ddY
Sex: Male
tissue: Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA ( GeneChip 3'IVT Express Kit User Manual, P/N702646 Rev.8, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 7G.
|
| Data processing |
CEL files were quantified with the Distribution Free Weighted method (DFW), using the statistical language R (R-2.7.2) and Bioconductor 2.2.
|
| |
|
| Submission date |
Sep 21, 2015 |
| Last update date |
Sep 06, 2016 |
| Contact name |
Fumika SHINOZAKI |
| E-mail(s) |
fp-shinozaki@newkast.or.jp
|
| Organization name |
Kanagawa Institute of Industrial Science and Technology
|
| Street address |
3-25-13 Tonomachi, Kawasaki-ku
|
| City |
Kawasaki |
| ZIP/Postal code |
210-0821 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE73264 |
Gene expression profiling of mice given orally a supplement with special amino acid composition of Vespa larval saliva origin (DW vs VAAM) |
| GSE73266 |
Gene expression profiling of mice given orally a supplement with special amino acid composition of Vespa larval saliva origin |
|
