|
| Status |
Public on Jul 12, 2016 |
| Title |
control-1 |
| Sample type |
SRA |
| |
|
| Source name |
embryos
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: S2
genotype/variation: control
|
| Growth protocol |
S2 cells were grown in Schneider’s Drosophila media (Gibco) with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
S2 cells were collected.Total RNA was isolated using ISOGEN II reagent (NIPPON GENE), according to the manufacturer’s protocol.
RNA-seq libraries were prepared using the TruSeq Stranded mRNA LT Sample Prep kit (Illumina)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
Sample 1
|
| Data processing |
Basecalls performed using Illumina MiSeq
RNA-seq reads were aligned to the Release 5 (dm3) genome assembly using Illumina MiSeq
Partek genomic suite was used to generate RPKMs. Quantification was performed using transcript-level and a significant signal was filtered by including at least one of the signal having more than one RPKM.
Genome_build: Release 5 (dm3)
Supplementary_files_format_and_content: RPKM
|
| |
|
| Submission date |
Sep 16, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
TAKEYA NAKAGAWA |
| E-mail(s) |
takeyanoemail@gmail.com
|
| Organization name |
Nagasaki unversity
|
| Department |
Biochemistry
|
| Street address |
1-12-4 sakamoto
|
| City |
Nagasaki |
| ZIP/Postal code |
852-8523 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16479 |
| Series (1) |
| GSE73098 |
Chameau Activates Transcription with Enhancer of Acetyltransferase Chameau (EAChm) in vitro |
|
| Relations |
| Reanalyzed by |
GSM3276731 |
| BioSample |
SAMN04090373 |
| SRA |
SRX1250572 |
