|
| Status |
Public on Sep 16, 2015 |
| Title |
Flag-D4 |
| Sample type |
SRA |
| |
|
| Source name |
MEF
|
| Organism |
Mus musculus |
| Characteristics |
strain background: ICR
cell type: Mouse Embryo Fibroblast (MEF)
genotype/variation: expressing Flag
time point: at day 4 of reprogramming
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Data processing |
Illumina Miseq software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie2-2.2.5 with parameters -p 8 --bowtie2 -sensitivity-level very_sensitive
Genome_build: mm10
Supplementary_files_format_and_content: text files include FPKM values for each Sample ...
|
| |
|
| Submission date |
Sep 15, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
zhongfu ying |
| E-mail(s) |
yingzhongfu@163.com
|
| Phone |
13560418156
|
| Organization name |
chinese academy of sciences
|
| Department |
stem cell research
|
| Lab |
stem cell
|
| Street address |
kaiyuan
|
| City |
guangzhou |
| State/province |
guangdong |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN04087873 |
| SRA |
SRX1246881 |
