|
| Status |
Public on Dec 15, 2015 |
| Title |
Diabetic 5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
27633_Diabetic_skeletal muscle
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skeletal muscle
metabolic class: Diabetic
|
| Treatment protocol |
Muscle tissue is collected under postprandial condition.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using Qiagen protocol.
|
| Label |
Cy5
|
| Label protocol |
Total RNA was converted to double-stranded cDNA and then into Cy-dye labeled cRNA using Agilent’s Low RNA Input Fluorescent Linear Amplification Kit. 750 ng of the labeled cRNA was fragmented and hybridized against Cy3-labeled Universal human reference (Stratagene, La Jolla, CA).
|
| |
|
| Channel 2 |
| Source name |
Universal human reference
|
| Organism |
Homo sapiens |
| Characteristics |
reference rna source: RNA from 10 human cell lines
|
| Biomaterial provider |
Stratagene, catalog number 740000
|
| Treatment protocol |
Muscle tissue is collected under postprandial condition.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using Qiagen protocol.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was converted to double-stranded cDNA and then into Cy-dye labeled cRNA using Agilent’s Low RNA Input Fluorescent Linear Amplification Kit. 750 ng of the labeled cRNA was fragmented and hybridized against Cy3-labeled Universal human reference (Stratagene, La Jolla, CA).
|
| |
|
| |
| Hybridization protocol |
Agilent In Situ Hybridization Kit Plus was used where Cy-dye labeled control and test samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, the arrays were washed twice, dried and then scanned.
|
| Scan protocol |
Scanned on an Agilent scanner, images were processed using Agilent Feature Extraction software version 10.7.
|
| Description |
Diabetic
|
| Data processing |
Agilent Feature Extraction software version 10.7 was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Sep 15, 2015 |
| Last update date |
Dec 15, 2015 |
| Contact name |
Rima Chaudhuri |
| E-mail(s) |
rima.chaudhuri@sydney.edu.au
|
| Organization name |
University of Sydney
|
| Department |
Charles Perkins Center
|
| Street address |
D17 Johns Hopkins Drive
|
| City |
Camperdown, Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE73034 |
Human skeletal muscle gene expression analysis on Lean, obese insulin sensitive, obese insulin resistant and obese Type II diabetic subjects |
| GSE73037 |
Cross-species Gene Expression Analysis Identifies a Novel Set of Genes Implicated in Human Insulin Sensitivity |
|
