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Sample GSM187531 Query DataSets for GSM187531
Status Public on May 08, 2007
Title Tumor samples from patient No.20 PT20U95B
Sample type RNA
 
Source name PT20, tumor samples from patient No.20
Organism Homo sapiens
Characteristics Tissue:primary prostate tumor
Tumor stage: T3a
Gleason Grade: 7
Treatment protocol Fresh prostate tissues were recovered immediately after surgical removal. Tissues were dissected/trimmed to obtain pure tumor then snap frozen in liquid nitrogen within 30 mintues of excision and stored in -80C untill RNA extraction. An H&E stained frozen section of each sample was evaluated by a pathologist, to determine epithelial and stromal content and verify the presence of tumor in the sample. Only tumor tissues with less than 30% of tromal components were selected.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiagen Rneasy kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U95B array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from primary prostate cancer
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200.
 
Submission date May 07, 2007
Last update date May 08, 2007
Contact name Federico Alberto Monzon
E-mail(s) famonzon@tmhs.org
Organization name The Methodist Hospital
Department Pathology
Lab Molecular Diagnostics
Street address 6565 Fannin St, MS205
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL92
Series (2)
GSE6606 Expression data from Primary Prostate Tumor
GSE6919 Expression Data from Normal and Prostate Tumor Tissues

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
41880_at 75.8
41881_at 53.6
41882_at 26.2
41883_at 13.6
41884_at 25.7
41885_at 58.1
41886_r_at 466
41887_at 8.9
41888_at 56.6
41889_at 28.4
41890_at 102.1
41891_at 30.5
41892_at 132.1
41893_at 18.2
41894_r_at 36.6
41895_at 198.4
41896_at 4
41897_at 86.2
41898_at 110.7
41899_at 73.2

Total number of rows: 12553

Table truncated, full table size 177 Kbytes.




Supplementary file Size Download File type/resource
GSM187531.CEL.gz 2.6 Mb (ftp)(http) CEL

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