|
| Status |
Public on Aug 20, 2015 |
| Title |
U3042 |
| Sample type |
genomic |
| |
|
| Source name |
U3042
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U3042
|
| Treatment protocol |
not applicable
|
| Growth protocol |
adherent cultures on laminin-coated dishes using neural stem cell media
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Dneasy blood and tissue kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
250 ng total genomic DNA was digested with the restriction enzyme Nsp I, then ligated to an Nsp I adaptor and further subjected to PCR amplification using a single primer. After fragmentation, the PCR products were labeled with a biotinylated nucleotide analogue using terminal deoxynucleotidyl transferase (TdT)
|
| |
|
| Hybridization protocol |
standard protocols for Affymetrix Cytoscan™ HD arrays (Affymetrix® Cytoscan User Guide (P/N 703038 Rev4.) Hybridized probes were captured by streptavidin-phycoerythrin conjugates using the GeneChip® Fluidics Station 450.
|
| Scan protocol |
scanned using the GeneChip® Scanner 3000 7G
|
| Data processing |
Data processing of CEL files was performed using Rawcopy software
|
| |
|
| Submission date |
Aug 19, 2015 |
| Last update date |
Aug 20, 2015 |
| Contact name |
Sven Nelander |
| Organization name |
Uppsala Universitet
|
| Street address |
Rudbecklab
|
| City |
Uppsala |
| ZIP/Postal code |
75217 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL16131 |
| Series (2) |
| GSE72209 |
CNA data from The human glioblastoma cell culture resource: validated cell models representing all molecular subtypes |
| GSE72219 |
The human glioblastoma cell culture resource |
|
