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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 31, 2016 |
| Title |
HSF1 ZT14 |
| Sample type |
SRA |
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| Source name |
Liver
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| Organism |
Mus musculus |
| Characteristics |
strain: C57/BL6 WT
feeding: ad libitum
technique: ChIP-seq
tissue: liver
chip antibody: HSF1
time point: ZT 14
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
For the HSF1 ChIP-Seq, chromatin preparation and chromatin immunoprecipitation were performed as in Rey et al., PLoS Biol 2011. The HSF1 polyclonal antibody is from Stressgen (Enzo Life Sciences). For each IP, 5ul of HSF1 antibody was used with 250ul of pre-cleared chromatin.
Libraries for ultra-high throughput sequencing were prepared with the ChIP-Seq DNA sample kit (Illumina) as recommended by the manufacturer.
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
ZT 14
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| Data processing |
All alignments were performed using the BBCF HTSstation (available at http://htsstation.epfl.ch, David et al., 2014)
Genome_build: mm9
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| Submission date |
Aug 17, 2015 |
| Last update date |
May 04, 2020 |
| Contact name |
Jonathan Aryeh Sobel |
| E-mail(s) |
jonathan.sobel@epfl.ch
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| Organization name |
EPFL
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| Department |
School of Life Sciences
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| Lab |
Felix Naef Lab
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| Street address |
EPFL SV IBI-SV UPNAE AAB 0 36 (Batiment AAB) Station 15
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| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
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| Platform ID |
GPL17021 |
| Series (2) |
| GSE60430 |
Regulatory logic of the coupled diurnal and feeding cycles in the mouse liver [DNase-seq, ChIP-seq] |
| GSE60578 |
Regulatory logic of the coupled diurnal and feeding cycles in the mouse liver |
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| Relations |
| BioSample |
SAMN03997109 |
| SRA |
SRX1155769 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1855803_HSF1_ZT14.bw |
120.3 Mb |
(ftp)(http) |
BW |
| GSM1855803_HSF1_ZT14_macs2_narrowPeak_annotated.txt.gz |
82.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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