|
| Status |
Public on Jul 25, 2015 |
| Title |
Female placenta Biol. Rep. 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
AFP2
|
| Organism |
Mus musculus |
| Characteristics |
group: APA
Sex: Female
strain: Swiss Webster
|
| Treatment protocol |
Female mice were rapidly sacrificed by cervical dislocation
|
| Growth protocol |
To obtain pregnancies, virgin females aged 3-months-old were mated with either 3-month-old (young paternal age group; 3 pairs) or 15-months-old (advanced paternal age group; 3 pairs) virgin sires. After that pregnancies were detected by the presence of a vaginal plug, males were removed and each dam was singly housed and allowed to deliver spontaneously. If no plug was detected after overnight male–female encountering, the female was removed and replaced by another, to avoid any possible effects of longer contact with the male. The morning, when the plug was detected was designed as Embryonic day 0.5 (E0.5).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using column-based Nucleospin miRNA (Machary-Nagel) according to manufacturer protocol
|
| Label |
Cy3
|
| Label protocol |
RNA was labeled using Two-Color Low Input Quick Amp Labeling Kits (Agilent) according to manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
CFP2
|
| Organism |
Mus musculus |
| Characteristics |
group: Control
Sex: Female
strain: Swiss Webster
|
| Treatment protocol |
Female mice were rapidly sacrificed by cervical dislocation
|
| Growth protocol |
To obtain pregnancies, virgin females aged 3-months-old were mated with either 3-month-old (young paternal age group; 3 pairs) or 15-months-old (advanced paternal age group; 3 pairs) virgin sires. After that pregnancies were detected by the presence of a vaginal plug, males were removed and each dam was singly housed and allowed to deliver spontaneously. If no plug was detected after overnight male–female encountering, the female was removed and replaced by another, to avoid any possible effects of longer contact with the male. The morning, when the plug was detected was designed as Embryonic day 0.5 (E0.5).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using column-based Nucleospin miRNA (Machary-Nagel) according to manufacturer protocol
|
| Label |
Cy5
|
| Label protocol |
RNA was labeled using Two-Color Low Input Quick Amp Labeling Kits (Agilent) according to manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
cRNA was hybridized using Gene Expression Hybridization Kit (Agilent). Hybridized microarrays were washed using standard Washing Buffer kit (Agilent). All procedures were performed according to manufacturer's protocol
|
| Scan protocol |
Data were extracted using G2565CA Microarray Scanner (Agilent, USA) and Agilent Feature Extraction Software (Agilent, USA) on default settings (GE2_1010_Sep10).
|
| Data processing |
Background correction method normexp was used and followed by loess method for within array normalization. Between array normalization was performed with the Aquantile method. Mean values of channels intensities were taken as an input and a median of background signal for background correction calculations.
|
| |
|
| Submission date |
Jul 24, 2015 |
| Last update date |
Jul 25, 2015 |
| Contact name |
Adrian Mateusz Stankiewicz |
| E-mail(s) |
adrianstankiewicz85@gmail.com
|
| Organization name |
Institute of Genetics and Animal Breeding of the Polish Academy of Sciences
|
| Department |
Animal Behaviour
|
| Street address |
Jastrzebiec, ul. Postepu 36A
|
| City |
Magdalenka |
| State/province |
Mazovia |
| ZIP/Postal code |
05-552 |
| Country |
Poland |
| |
|
| Platform ID |
GPL10333 |
| Series (1) |
| GSE71322 |
Placental transcriptomic pattern of murine advanced paternal age model of autism |
|
