|
| Status |
Public on Jan 06, 2016 |
| Title |
181837 |
| Sample type |
RNA |
| |
|
| Source name |
181837
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sex: Female
test article: Scl-Ab
dose: 3.0 mg/kg
vehicle: 0.004%Tween 20_9.0%sucrose_10.0mMsodium acetate/pH5
treatment status: Treated
cell type: Lining Cell
time point: 29.0 d
strain: SD, Rat Strain
|
| Treatment protocol |
Rats were administered by s.c. injection vehicle or 3 or 50 mg/kg/wk of a Scl-Ab once weekly
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Arcturus® PicoPure® RNA Isolation Kit (Life Technologies), with DNase treatment performed using QIAGEN’s RNase-Free DNase Set (QIAGEN Inc., Valencia, CA). RNA was eluted with 11 ?L of elution buffer from the RNA isolation kit
|
| Label |
biotin
|
| Label protocol |
Fifteen micrograms of amplified antisense mRNA (aRNA) was then biotinylated using the Turbo Labeling™ Kit-Biotin (Life Technologies) to generate microarray hybridization templates
|
| |
|
| Hybridization protocol |
Biotin-labeled aRNA products were fragmented and then hybridized to the Affymetrix GeneChip® Rat Genome 230 2.0 Array (Affymetrix, Santa Clara, CA) overnight at 45°C for 16 h in a GeneChip® Hybridization Oven 640
|
| Scan protocol |
Array chips were subsequently stained, washed, and scanned using a GeneChip® Fluidics Station 450, EukGE-WS2v5_450 script, and GCS 3000 7G with the AutoLoader (Affymetrix). Microarray hybridization was performed by Expression Analysis (Durham, NC).
|
| Description |
1.5 ng RNA was amplified using the Arcturus® RiboAmp® HS PLUS RNA Amplification Kit (Life Technologies).
|
| Data processing |
The scan files were processed using Expression Console (Affymetrix) for quality control and subsequent results were used in microarray data analysis. All statistical analyses were executed using the R statistical computing platform (version 3.0.1). Expression data were normalized using the Robust Multi-Array Average method implemented in the Bioconductor package affy (version 1.38.1
|
| |
|
| Submission date |
Jul 24, 2015 |
| Last update date |
Jan 06, 2016 |
| Contact name |
Scott Taylor |
| E-mail(s) |
Scott.Taylor@Amgen.com
|
| Organization name |
Amgen Inc.
|
| Street address |
One Amgen Center Drive
|
| City |
Thousand Oaks |
| State/province |
CA |
| ZIP/Postal code |
91320-1799 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE71306 |
Scl-Ab: Exploratory 26-Week Subcutaneous Toxicology Study in the Aged Ovariectomized Female Sprague Dawley Rat with an 18-week Recovery [vertebrae] |
|
