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Status |
Public on Jan 06, 2016 |
Title |
181837 |
Sample type |
RNA |
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Source name |
181837
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Organism |
Rattus norvegicus |
Characteristics |
Sex: Female test article: Scl-Ab dose: 3.0 mg/kg vehicle: 0.004%Tween 20_9.0%sucrose_10.0mMsodium acetate/pH5 treatment status: Treated cell type: Lining Cell time point: 29.0 d strain: SD, Rat Strain
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Treatment protocol |
Rats were administered by s.c. injection vehicle or 3 or 50 mg/kg/wk of a Scl-Ab once weekly
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Arcturus® PicoPure® RNA Isolation Kit (Life Technologies), with DNase treatment performed using QIAGEN’s RNase-Free DNase Set (QIAGEN Inc., Valencia, CA). RNA was eluted with 11 ?L of elution buffer from the RNA isolation kit
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Label |
biotin
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Label protocol |
Fifteen micrograms of amplified antisense mRNA (aRNA) was then biotinylated using the Turbo Labeling™ Kit-Biotin (Life Technologies) to generate microarray hybridization templates
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Hybridization protocol |
Biotin-labeled aRNA products were fragmented and then hybridized to the Affymetrix GeneChip® Rat Genome 230 2.0 Array (Affymetrix, Santa Clara, CA) overnight at 45°C for 16 h in a GeneChip® Hybridization Oven 640
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Scan protocol |
Array chips were subsequently stained, washed, and scanned using a GeneChip® Fluidics Station 450, EukGE-WS2v5_450 script, and GCS 3000 7G with the AutoLoader (Affymetrix). Microarray hybridization was performed by Expression Analysis (Durham, NC).
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Description |
1.5 ng RNA was amplified using the Arcturus® RiboAmp® HS PLUS RNA Amplification Kit (Life Technologies).
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Data processing |
The scan files were processed using Expression Console (Affymetrix) for quality control and subsequent results were used in microarray data analysis. All statistical analyses were executed using the R statistical computing platform (version 3.0.1). Expression data were normalized using the Robust Multi-Array Average method implemented in the Bioconductor package affy (version 1.38.1
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Submission date |
Jul 24, 2015 |
Last update date |
Jan 06, 2016 |
Contact name |
Scott Taylor |
E-mail(s) |
Scott.Taylor@Amgen.com
|
Organization name |
Amgen Inc.
|
Street address |
One Amgen Center Drive
|
City |
Thousand Oaks |
State/province |
CA |
ZIP/Postal code |
91320-1799 |
Country |
USA |
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Platform ID |
GPL1355 |
Series (1) |
GSE71306 |
Scl-Ab: Exploratory 26-Week Subcutaneous Toxicology Study in the Aged Ovariectomized Female Sprague Dawley Rat with an 18-week Recovery [vertebrae] |
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