|
| Status |
Public on Aug 31, 2016 |
| Title |
C_38736_27 |
| Sample type |
RNA |
| |
|
| Source name |
prostate cancer
|
| Organism |
Homo sapiens |
| Characteristics |
gleason_score: 4+3
ethnicity: African-American
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted following the manufacturer's recommendations.
|
| Label |
cy3
|
| Label protocol |
The cRNA labeling was performed according to Protocol from Agilent Technologies Inc. (Santa Clara, CA) using the Agilent Quick Amp Labeling Kit (Agilent Technologies, Palo Alto, CA) following the two-colour manufacturer's protocol.
|
| |
|
| Hybridization protocol |
cRNA samples were hybridized to an Agilent Technologies Human Gene Expression 8x60K Microarray Agilent Technologies). The hybridization slides were washed, stabilized, dried, and immediately scanned.
|
| Scan protocol |
Scanned with an Agilent Technologies Microarray Scanner using default parameters. Each sample represents one channel of a dual-channel array.
|
| Data processing |
Expression arrays were processed using BioConductor (one color arrays, Quantile normalization). Also we combated the dataset by patient (as the “batch” variable).
|
| |
|
| Submission date |
Jul 16, 2015 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Chad Creighton |
| E-mail(s) |
creighto@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Biostatistics, Ducan Cancer Center
|
| Street address |
One Baylor Plaza, Mail Stop: BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL16699 |
| Series (1) |
| GSE71016 |
Gene expression profiling of African-American prostate cancer |
|
