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Sample GSM174884 Query DataSets for GSM174884
Status Public on Jan 01, 2008
Title E mock 4 dpi rep 1 with array type rice from Affymetrix
Sample type RNA
Source name E_mock_4dpi_rep1
Organism Oryza sativa
Characteristics Oriza sativa - dev.stage (Boyes et al. Plant Cell 2001):last emerged leaf (3d leaf)
Treatment protocol Name:infection_rice_mock - environmental treatment - pathogen infection,magnaporthe grisea :. Plants were inoculated with gelatine 0.5% (Berruyer et al, TAG 2005)
Growth protocol leaf - Growth on soil- 60% humidity- 28degreeC day/25degreeC dark- 12h day
Extracted molecule total RNA
Extraction protocol E_mock_4dpi_rep1:20.5ug.
Label Biotin
Label protocol labelling Biotin direct, amplification=yes, RNAa 20 ug.
Hybridization protocol labelled extract quantity: 12ug
Scan protocol GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
Description Whereas plant disease resistance is well documented at the transcriptomic level, very few analyses have been conducted, especially in cereals, on plant disease susceptibility. In addition, in rice, susceptibility is often not well defined at the phenotypical level and some interactions termed as compatible can be in fact a mixture of compatible and incompatible interactions. We designed a specific experiment in which the interaction between rice and its fungal pathogen Magnaporthe grisea was truly compatible (complete absence of background resisatnce) in order to identify genes that would be differentially regulated in such condition. These genes may potentially represent targets for effectors of the pathogen.
Data processing Normalized data by GCRMA
Submission date Mar 13, 2007
Last update date Mar 13, 2007
Contact name sandrine balzergue
Organization name INRA
Street address Rue G. Morel
City Beaucouze
ZIP/Postal code 49000
Country France
Platform ID GPL2025
Series (1)
GSE7256 Identification of rice genes differentially expressed upon virulent infection by Magnaporthe grisea

Data table header descriptions
ID_REF ID number
VALUE Normalized data by GCRMA

Data table
AFFX-BioB-3_at 8.11
AFFX-BioB-5_at 7.55
AFFX-BioB-M_at 7.51
AFFX-BioC-3_at 9.40
AFFX-BioC-5_at 9.13
AFFX-BioDn-3_at 11.94
AFFX-BioDn-5_at 10.68
AFFX-CreX-3_at 14.02
AFFX-CreX-5_at 14.09
AFFX-DapX-3_at 12.33
AFFX-DapX-5_at 10.83
AFFX-DapX-M_at 11.50
AFFX-LysX-3_at 9.22
AFFX-LysX-5_at 7.32
AFFX-LysX-M_at 7.69
AFFX-Mgr-actin-3_at 2.64
AFFX-Mgr-actin-5_at 2.57
AFFX-Mgr-actin-M_at 2.24
AFFX-Mgr-ef1a-3_at 3.25
AFFX-Mgr-ef1a-3_x_at 2.33

Total number of rows: 57381

Table truncated, full table size 1345 Kbytes.

Supplementary file Size Download File type/resource
GSM174884.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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