|
| Status |
Public on Feb 22, 2007 |
| Title |
Rust Innoculated, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Glycine max rust inoculated leaf (Treatment)
|
| Organism |
Glycine max |
| Characteristics |
5601T unknown reaction to Asian soybean rust
Leaf samples collected 72 hours after inoculation from V2 growth stage)
|
| Treatment protocol |
Control plants at V2 stage were not inoculated rather moved to an adjacent growth chamber with congruent growing environment).
|
| Growth protocol |
Three plants per pot were maintained and growing environment consisted of 28/20 degree celsius, 14/10 photoperiod with high humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Soybean Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 7G scanner.
|
| Description |
Gene expression analysis in soybean in response to Phakopsora pachyrhizii.
|
| Data processing |
The image was scanned using GCOS software and the corresponding CEL file generated. Array Assist was used to summarize the signal values using the GCRMA algorithm.
|
| |
|
| Submission date |
Feb 22, 2007 |
| Last update date |
Feb 22, 2007 |
| Contact name |
Dilip Raj Panthee |
| E-mail(s) |
dpanthee@utk.edu
|
| Phone |
(865)974 1903
|
| Organization name |
University of Tennessee
|
| Department |
Plant Sciences
|
| Street address |
2431 Joe Johnson Dr.
|
| City |
Knoxville |
| State/province |
TN |
| ZIP/Postal code |
37996 |
| Country |
USA |
| |
|
| Platform ID |
GPL4592 |
| Series (1) |
| GSE7108 |
Gene expression analysis in soybean with respect to Pahakopsora pachyrhizii at V2 growth stage |
|
