|
| Status |
Public on Jul 31, 2015 |
| Title |
A549_Low attachment 5days_2 |
| Sample type |
RNA |
| |
|
| Source name |
A549 cells_low-attachment plate with normal medium
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
|
| Treatment protocol |
A549 cells were cultured for 5 days in attachment or low-attachment 96 well plate with 10 % FBS/DMEM supplemented with FP001 (0.015 w/v%) or Milli-Q water.
|
| Growth protocol |
Non-small lung cancer cells A549 were cultured in DMEM supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed with Quiagen RNeasy mini kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (3' IVT Express Kit User Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Gene 2.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with Hybridization, Wash and Stain Kit.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 scanner.
|
| Description |
Gene expression data from A549 cells which were isolated from cultured A549 cells.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 500.
|
| |
|
| Submission date |
Jun 02, 2015 |
| Last update date |
Jul 31, 2015 |
| Contact name |
Ayako Otani |
| E-mail(s) |
otania@nissanchem.co.jp
|
| Organization name |
Nissan Chemical Industries
|
| Department |
Medical Materials group
|
| Street address |
1470 Shiraoka
|
| City |
Shiraoka |
| State/province |
Saitama |
| ZIP/Postal code |
349-0294 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE69477 |
Gene expression profile of A549 cells cultured with novel 3D cell culture method |
|
