 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 03, 2015 |
| Title |
pelo1/+ rep1 |
| Sample type |
SRA |
| |
|
| Source name |
ovary
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: pelo1/+
tissue: ovary
age: 3-5 days old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ovaries were manually dissected in ice-cold PBS. Total RNA were isolated using Trizol. 18-30nt RNAs were purified according to RNA oligo markers.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
18-30nt small RNA
|
| Data processing |
Reads which passed filter were mapped to rRNA, tRNA, snoRNA, miRNA, transposon, nonLTR, LTR, TRF and Drosophila melanogaster whole genome (BDGP5.25) using bowtie v0.12.7 with parameters -q -p 20 -m 1 -v 2, respectively. The miRNA reference was download from miRBase, and other resource were downloaded from Repbase.
|
| |
|
| Submission date |
Jun 02, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
huanwei huang |
| E-mail(s) |
hhw2436@gmail.com
|
| Organization name |
NIBS
|
| Street address |
7 Science Park Rd., ZGC life science park
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platform ID |
GPL9061 |
| Series (1) |
| GSE69468 |
Small RNA profile of pelo1/+ and peloPB60/1 ovaries |
|
| Relations |
| BioSample |
SAMN03757463 |
| SRA |
SRX1046507 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1701584_wt.miRNA.rawcounts.txt.gz |
1.0 Kb |
(ftp)(http) |
TXT |
| GSM1701584_wt.piwiRNA.rawcounts.txt.gz |
9.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
