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Sample GSM1697618 Query DataSets for GSM1697618
Status Public on Sep 27, 2015
Title iPSCs, Nos3-/- mice, Day 0, 6
Sample type RNA
 
Source name iPSCs, Nos3-/- mice, Day 0
Organism Mus musculus
Characteristics cell type: iPSCs
genotype/variation: Nos3-/-
strain: B6.129P2-Nos3tm1unc/J
time point: Day 0
Treatment protocol iPSCs were generated via Sendai viral reprogramming of CD1 and Nos3-/- tail-tip fibroblasts. Cells were cultured on inactive MEF feeders in the pluripotent state and spontaneously differentiated via aggrewell-mediated EB formation to D11.
Growth protocol The study samples consisted of iPSCs (D0) reprogrammed from tail-tip fibroblasts of adult male wild type CD1 and Nos3-/- mice and differentiated embryoid bodies (D11).
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description iPSCs, Nos3-/- mice, Day 0
Data processing The data were analyzed with R/Bioconductor using RMA.
 
Submission date May 28, 2015
Last update date Sep 27, 2015
Contact name XING LI
E-mail(s) li.xing@mayo.edu
Organization name Mayo Clinic
Department Health Sciences Research
Street address 200 1st street SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platform ID GPL1261
Series (2)
GSE69316 Nos3-/- iPSCs model concordant signatures of in utero cardiac pathogenesis [iPSCs]
GSE69317 Nos3-/- iPSCs model concordant signatures of in utero cardiac pathogenesis

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
1415670_at 9.73
1415671_at 11.22
1415672_at 12.06
1415673_at 11.36
1415674_a_at 10.95
1415675_at 9.58
1415676_a_at 12.99
1415677_at 9.74
1415678_at 11.14
1415679_at 11.78
1415680_at 10.27
1415681_at 10.98
1415682_at 9.15
1415683_at 12.19
1415684_at 10.16
1415685_at 10.40
1415686_at 9.80
1415687_a_at 11.50
1415688_at 11.05
1415689_s_at 9.55

Total number of rows: 45101

Table truncated, full table size 727 Kbytes.




Supplementary file Size Download File type/resource
GSM1697618_Nel010385_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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