|
| Status |
Public on Jul 31, 2015 |
| Title |
dnMPL_GFP neg_2 |
| Sample type |
RNA |
| |
|
| Source name |
GFP- LSK cells from C57Bl/6 mice 8 wks after Tx with dnMPL transduced LSK cells
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL6
transplanted with: dnMPL transduced LSK cells
cell type: GFP negative Lin-Sca1+cKit+ (LSK) cells
|
| Treatment protocol |
Transduction with retroviral supernatants 48h after isolation; transplantation into syngenic mice. FACS-isolation 8 weeks after transplantation
|
| Growth protocol |
transgene positive and negative Lin-Sca1+cKit+ (LSK) cells were isolated from dnMpl and control transplanted mice by fluorescent activated cell sorting. For each sample, BM of 2-5 mice were pooled.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Micro Kit (Qiagen GmbH, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
RNA was reverse transcribed, amplified and labelled using the Nugen Ovation Pico and Encore biotin kits (Nugen Technologies, AC Bemmel, Netherlands).
|
| |
|
| Hybridization protocol |
amplified, fragmented, biotin labeled aRNA was hybridized for 18 hr at 45C on Affymetrix GeneChip Mouse 430 2.0. Chips were washed and stained in the Affymetrix Fluidics Station 450 according to manufacturers instructions
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
SAMPLE 5
Gene expression data from GFP-negative LSK cells from dnMPL transplanted mice
|
| Data processing |
RMA (Robust Multichip Average; Bioconductor)
|
| |
|
| Submission date |
May 26, 2015 |
| Last update date |
Jul 31, 2015 |
| Contact name |
Adrian Schwarzer |
| E-mail(s) |
schwarzer.adrian@mh-hannover.de
|
| Phone |
00495115325143
|
| Organization name |
Hannover Medical School
|
| Department |
Experimental Hematology
|
| Street address |
Carl-Neuberg-Str. 1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE69209 |
Gene expression changes induced by expression of dMPL in murine bone haematopoietic progenitor cells |
|
