|
| Status |
Public on May 21, 2015 |
| Title |
Cont1 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2_siRNA against GL3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HepG2
cell type: human hepatoma cells
transfected with: siRNA against GL3
|
| Treatment protocol |
For the knockdown experiments, specific siRNAs were introduced to the cells using RNAiMAX reagent (Invitrogen) when they were approximately 50% confluent.
|
| Growth protocol |
HepG2 cells were cultured in high glucose (25mM D-glucose) Dulbecco-modified eagle’s medium (DMEM) (Sigma) supplemented with 10% (v/v) heat-inactivated fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After cultured for 4 days, semiconfluent cells were harvested RNA extraction using RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
GeneChip 3' IVT Express Kit
|
| |
|
| Hybridization protocol |
GeneChip Hybridization, Wash, and Stain Kit
|
| Scan protocol |
GCS3000 Scanner
|
| Description |
Cont1_(HG-U133_Plus_2) .CEL(normalized)
|
| Data processing |
The data were analyzed with Affymetrix Genechip Command Console software (CEL files) using Affymetrix default analysis settings and global scaling, and baseline transformed to the median of control 2 samples. GeneSpring software (v.13.0) was used for summarization by RMA algorithm and baseline transformation (median shift).
|
| |
|
| Submission date |
May 20, 2015 |
| Last update date |
May 27, 2015 |
| Contact name |
Akihisa Sakamoto |
| Organization name |
Kumamoto University
|
| Department |
Institute of Molecular Embryology and Genetics
|
| Lab |
Department of Medical Cell Biology
|
| Street address |
2-2-1 Honjo, Chuo-ku
|
| City |
Kumamoto |
| ZIP/Postal code |
860-0811 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE69075 |
Role of histone demethylase LSD1 in hepatic cancer metabolism |
|
