|
| Status |
Public on May 21, 2015 |
| Title |
PTPRZ1 knocking-down glioblastoma stem cells |
| Sample type |
RNA |
| |
|
| Source name |
PTPRZ1 knocking-down glioblastoma stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: glioblastoma stem cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The PTPRZ1 knocking-down glioblastoma stem cells and control cells were used for RNA isolatation using Primescript RT Master Mix (Takara) according to the manufacture's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20ug total RNA (Cat. AM1792, Ambion).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 with constant rotation at the speed of 60 rpm. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
PTPRZ1 knocking down cells with disrupting efficiency confirmed by qRT-PCR
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method, following the manufacture's protocol. Differentially expressed genes were identified when the gene expression ratio between the control group and KD group was more than 2-fold.
|
| |
|
| Submission date |
May 18, 2015 |
| Last update date |
May 21, 2015 |
| Contact name |
Yu Shi |
| E-mail(s) |
cadet_toutou@126.com
|
| Organization name |
Institute of Pathology and Southwest Cancer Center
|
| Department |
Southwest Hospital
|
| Lab |
Key Laboratory of Tumor Immunopathology of Ministry of Education of China
|
| Street address |
Gao-tan Yan Street
|
| City |
Chongqing |
| State/province |
Chongqing |
| ZIP/Postal code |
400038 |
| Country |
China |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE68994 |
PTPRZ1 disruption-mediated gene expression changes in human glioblastoma stem cells [HG-U133_Plus_2] |
| GSE69081 |
Gene expression profiles of PTPRZ1-knocking-down human glioblastoma stem cells |
|
