|
| Status |
Public on Aug 01, 2015 |
| Title |
HuEx_10_ES-7 |
| Sample type |
RNA |
| |
|
| Source name |
human cell line
|
| Organism |
Homo sapiens |
| Characteristics |
type: Ewing Sarcoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were grown in flasks to approximately 70% confluency, detached with trypsin, and then total RNA extracted using Qiagen miRNeasy mini-kit according to manufacturers instructions. Quality of RNA was assessed using an Agilent 2100 Bioanalyzer and all samples were of good quality with RIN# > 8.
|
| Label |
biotin
|
| Label protocol |
Total RNA (100 ng) was reverse transcribed and amplified using Ambion WT expression kit following manufacturer’s suggested protocol. Sense strand cDNA was fragmented and labeled using Affymetrix WT terminal labeling kit.
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human Exon 1.0 ST Arrays (Affymetrix) in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs. Wash and stain were performed on Affymetrix Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned on Affymetrix GeneChip scanner 3000 7G.
|
| Data processing |
Probe set summarization and normalization with the core annotated probes was performed by robust multi-array averaging (RMA, including background subtraction, summarization by median polish and quantile normalization) using the R package aroma.affymetrix.
|
| |
|
| Submission date |
May 05, 2015 |
| Last update date |
Aug 01, 2015 |
| Contact name |
Eric C Polley |
| E-mail(s) |
eric.polley@nih.gov
|
| Organization name |
National Cancer Institute
|
| Department |
Biometric Research Branch
|
| Street address |
9609 Medical Center Drive, Rm 5W118
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL11028 |
| Series (2) |
| GSE68591 |
Exon expression for NCI Sarcoma cell line panel |
| GSE69524 |
NCI Sarcoma Cell Line Panel |
|
