|
| Status |
Public on Jun 15, 2015 |
| Title |
clone10 |
| Sample type |
RNA |
| |
|
| Source name |
preadipocyte_BAT
|
| Organism |
Homo sapiens |
| Characteristics |
subject: 1
tissue: BAT
mature adipocyte ucp1 (measured by luciferase reporter system): 42.87
|
| Growth protocol |
Immortalized progenitor cells were plated and grown in DMEM/H medium supplemented with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from clonal cell lines using Direct-zol RNA MiniPrep kit (Zymo Research) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was synthesized, purified and fragmented using GeneChip 3’IVT Express Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
10ug of biotinylated cRNA were hybridized for 16hr at 45C to the array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Data processing |
Microarray data were normalized using robust multi-array average (RMA)
|
| |
|
| Submission date |
May 05, 2015 |
| Last update date |
Jun 23, 2015 |
| Contact name |
Jonathan M Dreyfuss |
| Organization name |
Joslin Diabetes Center
|
| Department |
Bioinformatics & Biostatistics Core
|
| Street address |
1 Joslin Pl
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE68544 |
Human brown and white preadipocyes clonal dynamics predict themorgenic potential and function |
|
