|
| Status |
Public on Apr 30, 2019 |
| Title |
Rh30_siNR4A1/2_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Rh30_siNR4A1/2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Rh30
cell type: Rhabdomyosarcoma
|
| Treatment protocol |
Rh30 rhabdomyosarcoma cells were transfected for 48 hours with 25 nM of Dharmacon ON-TARGETplus SMARTpool NR4A-specific siRNA or non-targeting siRNA alone or in combination (total of 75nM siRNA) using 6μL Lipofectamine RNAiMAX per well of a 6-well plate
|
| Growth protocol |
Rh30 rhabdomyosarcoma cells were cultured in RPMI-1640 medium containing 10% fetal bovine serum at 37°C, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Promega simplyRNA kit and Maxwell-16 following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA were labeled (Cy3) according to Agilent's labeling protocol
|
| |
|
| Hybridization protocol |
Microarray hybridization was performed at 65°C for 18 hours in Agilent's microarray hybridization chambers, followed by wash procedures according to the manufacturer’s recommended protocols
|
| Scan protocol |
Microarray was scanned in an Agilent scanner at 3μm resolution, and the array data was extracted with Agilent feature extraction software using the GE1_107_Sep09 protocol
|
| Description |
Gene expression after 48hr cotransfection with 25nM siRNA against NR4A1, 25nM siRNA against NR4A2, and 25nM non-targeting siRNA, biological replicate 3
|
| Data processing |
Agilent Feature Extraction Software (version 10.5.1.1) was used along with LOWESS normalization
|
| |
|
| Submission date |
Apr 30, 2015 |
| Last update date |
Apr 30, 2019 |
| Contact name |
Yong-Dong Wang |
| E-mail(s) |
michael.wang@stjude.org
|
| Phone |
9015954224
|
| Organization name |
St. Jude Children's Research Hospital
|
| Street address |
262 Danny Thomas Pl, MS1135
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38134 |
| Country |
USA |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE68458 |
Analysis of NR4A-specific gene expression in human alveolar rhabdomyosarcoma in response to specific gene knockdown |
|
