strain: C57BL gender: male tissue: liver treatment short name: H2O treatment full name: Water treatment duration [days]: 56 treatment dose: 0 % (w/v) group: H2O 56d
Treatment protocol
After 28 (or 56) days treatment, mice were sacrificed by a rising concentration of carbon dioxide, and subject to necropsy to remove the liver, which was rinsed in sterile phosphate-buffered saline, snap-frozen in liquid nitrogen and stored at -80oC.
Growth protocol
Adult male mice (mus muculus) on a C57BL/6J background were treated with phenobarbital (0.05%w/v, drinking water) for 28 or 56d; CITCO for 28d (daily, 10mg/kg, intraperitoneal); WY14,643 for 28d (daily, 100mg/kg, oral gavage); piperonyl butoxide for 28d (daily, 600mg/kg, intraperitoneal); pioglitazone for 28d (daily, 100mg/kg, oral gavage); methapyrilene for 28d (1000ppm, in food); 1,4-Dichlorobenzene for 28d (daily, 600mg/kg, oral gavage); cyproterone acetate for 28d (daily, 16omg/kg, oral gavage).
Extracted molecule
total RNA
Extraction protocol
Liver tissue was homogenized and RNA isolation was performed with Qiazol extraction (Qiagen, Hilden,Germany).
Label
biotin
Label protocol
Labeling according to 3'IVT Express Kit User Manual Affymetrix
Hybridization protocol
cRNA synthesis, target hybridization, washing, staining and subsequent probe array scanning was done according to the manufacturer's instructions (3'IVT Express Kit User Manual Affymetrix)
Transcriptional profiling of mouse liver after short-term in vivo exposure (up to 56 days) of C57BL-mice to carcinogenic and non-carcinogenic chemicals
