|
| Status |
Public on Nov 02, 2015 |
| Title |
WT3 |
| Sample type |
SRA |
| |
|
| Source name |
Decapsulated testis
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6|129/Sv|FVB/N Mixed BKG
tissue: Testis
age: post natal day 21
genotype: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Testes were decapsulated and flash frozen in liquid nitrogen. Total RNA was extracted using Qiagen RNEasy Midi Kit columns and reagents; Cat 75142
Libraries were prepared according to Illumina's instructions using the Truseq RNA-seq protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Basecalls performed using CASAVA version 1.8.2
Reads were mapped to the reference genome using STAR 2.4 with --outFilterMultimapNmax 1
Raw count measurements were obtained with featureCounts 1.4.6 keeping only unambiguous reads
Genome_build: GRCm38.70
Supplementary_files_format_and_content: Raw counts measurements
|
| |
|
| Submission date |
Apr 27, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Sylvain LEMEILLE |
| Organization name |
University of Geneva Medical School
|
| Department |
Department of Pathology and Immunology
|
| Street address |
1 rue Michel-Servet
|
| City |
Geneva |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL17021 |
| Series (2) |
| GSE63130 |
Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes |
| GSE68284 |
Next Generation Sequencing Analysis of Wild Type and Rfx2-/- Testicular Transcriptomes [RNA-Seq P21] |
|
| Relations |
| BioSample |
SAMN03568985 |
| SRA |
SRX1011093 |
