|
| Status |
Public on Mar 24, 2016 |
| Title |
Batch1_Mouse-13.5 D_Mandible_Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryo stage E13.5
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
tissue: Distal bone
developmental stage: embryo
age: E13.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from tissues using RNeasy mini kit (Qiagen Valenica, CA)
|
| Label |
biotin
|
| Label protocol |
Starting from 3 µg total RNA, labeled cRNA was prepared using the Qiagen Microarray cDNA Synthesis, Microarray RNA Target Synthesis (T7) and Microarray Target Purification Kit, according to the manufacturer's instructions
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 µg of cRNA were hybridized for 16hr on Affymetrix Mouse 430_2 (gene version) as recommended by manufacturer
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000A.
|
| Description |
Gene expression
|
| Data processing |
RMA (Roboust Multi_array average) normalization was used
|
| |
|
| Submission date |
Apr 17, 2015 |
| Last update date |
Apr 22, 2016 |
| Contact name |
Vasu Punj |
| E-mail(s) |
vasupunj@gmail.com
|
| Organization name |
USC
|
| Street address |
Biggy Street
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE67985 |
To integrated functional genomics and craniofacial morphogenesis with in FaceBase Consortium |
|
