|
| Status |
Public on Mar 31, 2015 |
| Title |
Mouse 6h Ischemia Core 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
6h Saline Ischemia Core Cy3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
brain region: ischemia core
|
| Treatment protocol |
Mice were first anesthetized with 4% sevoflurane (induction) and 2 % sevoflurane (maintenance) in a 30% O2 and 70% N2O gas mixture via a face mask, followed by an incision in the cervical skin, opening of salivary gland, resulting in visualization of the right common carotid artery. A midline cervical incision was made to expose the external carotid artery, and using intraluminal filament technique the PMCAO model was generated. PACAP38 (1 l containing 1 pmol) or 1 µl of saline (0.9% NaCl, as control) was injected intracerebroventrically, immediately after PMCAO. PACAP38 (Peptide Institute Inc., Osaka, Japan; supplier temperature was -20 ºC) was dissolved at 10-5M concentration by saline, and stored at -80 ºC. PACAP test solution (for injection) was diluted ×10 times with 0.9% NaCl just before use. After injections, the animals were returned to their cages. A total of four groups were prepared
|
| Growth protocol |
Animal care and experimental procedures were used as approved by the Institutional Animal Care and Use Committee of Showa University (School of Medicine), Tokyo, Japan. Thirty male mice (C57BL/6J) of 9-weeks-old (25 ~ 35 g body weight) were purchased from Charles River (Kanagawa, Japan). Mice were housed at the Animal Institution in Showa University in acrylic cages (8 mice/cage) maintained at 23 ºC with a standard 12 h light/dark cycle, optimum humidity, and temperature control. Animals were given access to tap water and laboratory chow ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
kit: QIAGEN, Rneasy Mini
|
| Label |
Cy3
|
| Label protocol |
Manual: Low Input Quick Amp Labeling Kit, Two-Color (Agilent)
|
| |
|
| Channel 2 |
| Source name |
6h Saline Healthy Core Cy5
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
brain region: healthy core
|
| Treatment protocol |
Mice were first anesthetized with 4% sevoflurane (induction) and 2 % sevoflurane (maintenance) in a 30% O2 and 70% N2O gas mixture via a face mask, followed by an incision in the cervical skin, opening of salivary gland, resulting in visualization of the right common carotid artery. A midline cervical incision was made to expose the external carotid artery, and using intraluminal filament technique the PMCAO model was generated. PACAP38 (1 l containing 1 pmol) or 1 µl of saline (0.9% NaCl, as control) was injected intracerebroventrically, immediately after PMCAO. PACAP38 (Peptide Institute Inc., Osaka, Japan; supplier temperature was -20 ºC) was dissolved at 10-5M concentration by saline, and stored at -80 ºC. PACAP test solution (for injection) was diluted ×10 times with 0.9% NaCl just before use. After injections, the animals were returned to their cages. A total of four groups were prepared
|
| Growth protocol |
Animal care and experimental procedures were used as approved by the Institutional Animal Care and Use Committee of Showa University (School of Medicine), Tokyo, Japan. Thirty male mice (C57BL/6J) of 9-weeks-old (25 ~ 35 g body weight) were purchased from Charles River (Kanagawa, Japan). Mice were housed at the Animal Institution in Showa University in acrylic cages (8 mice/cage) maintained at 23 ºC with a standard 12 h light/dark cycle, optimum humidity, and temperature control. Animals were given access to tap water and laboratory chow ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
kit: QIAGEN, Rneasy Mini
|
| Label |
Cy5
|
| Label protocol |
Manual: Low Input Quick Amp Labeling Kit, Two-Color (Agilent)
|
| |
|
| |
| Hybridization protocol |
hybridization_Kit: in situ hybridization plus
|
| Scan protocol |
Scanner: Agilent DNA microarray Scanner G2505C
|
| Data processing |
Software: Feature Extraction ver 11.0.1.1
|
| |
|
| Submission date |
Mar 30, 2015 |
| Last update date |
Mar 31, 2015 |
| Contact name |
RANDEEP RAKWAL |
| E-mail(s) |
plantproteomics@gmail.com
|
| Phone |
+81-(0)90-1853-7875
|
| Organization name |
University of Tsukuba
|
| Department |
Institute of Health and Sport Sciences
|
| Lab |
Global Sport Innovation (GSI) 403
|
| Street address |
1-1-1 Tennodai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8574 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE67421 |
Bioinformatics Analysis of the Permanent Middle Cerebral Artery Occlusion (PMCAO) Mouse Model Brain Regions Gene Profiles for Unraveling Progression of the Ischemic Core |
|
