|
| Status |
Public on Oct 01, 2015 |
| Title |
Fetal liver, Rb1 KO, 24h, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Fetal liver, Rb1 KO, 24 hour
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
developmental stage: E14.5 fetal
genotype: Rb1 KO
culture time: 24 hour
|
| Treatment protocol |
The samples were untreated.
|
| Growth protocol |
Fetal liver cells grown in culture in complete medium (30% fetal bovine serum, 1% deionized bovine serum albumin, 0.001% monothioglycerol, 2 mM glutamine, and penicillin-streptomycin in Iscove’s modified Dulbecco medium) at 37C for 0, 24, and 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNazol Bee extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA (1-10ug) was processed in the Hartwell Center microarray core according to the Affymetrix eukaryote one-cycle target labeling protocol (P/N 701025 Rev.6).
|
| |
|
| Hybridization protocol |
Biotin-labeled cRNA (15ug) was hybridized overnight at 45C to the Mouse Genome 430 2.0 GeneChip array
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Rb1 KO-8
|
| Data processing |
Expression values were summarized using the MAS5 algorithm as implemented in the GCOS v1.4 software. Signals were normalized for each array by scaling to a 2% trimmed mean of 500.
|
| |
|
| Submission date |
Mar 25, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Paul A Ney |
| E-mail(s) |
paulney1@gmail.com
|
| Organization name |
New York Blood Center
|
| Street address |
310 E 67th St
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE67278 |
Expression data from murine fetal liver cells in culture, time course, combinations of RB1 and p107 knockouts |
| GSE67285 |
Repression by RB1 characterizes genes involved in the penultimate stage of erythroid differentiation |
|
