|
| Status |
Public on Dec 10, 2015 |
| Title |
shACLY-#2_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
H1975 cells, shshACLY, 24h hypoxia
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1975
cell type: lung cancer cells
shRNA: shACLY
growth condition: hypoxia for 24hr
|
| Treatment protocol |
Cells in 10cm dishes were serum starved for 24 hours, then transferred to 1% oxygen for an additional 24 hours. Immediately washed with cold PBS, scraped, and frozen at -80C.
|
| Growth protocol |
H1975 lung cancer cells with stable knockdown of ACC1, ACLY or ETV4 after 24 hours of serum starvation (0.5% FBS) followed by 24 hours of hypoxia (1% O2).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN's RNeasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
Ambion MessageAmp Premier Kit.
|
| |
|
| Hybridization protocol |
Affymetrix Human Genome U133A 2.0 Array following the Affymetrix standard protocol.
|
| Scan protocol |
Affymetrix GeneChip Scanner 7G.
|
| Description |
ACLY-285-C
|
| Data processing |
Expression Console was used to generate the RMA file.
|
| |
|
| Submission date |
Mar 09, 2015 |
| Last update date |
Dec 10, 2015 |
| Contact name |
Jen-Tsan Chi |
| E-mail(s) |
jentsan.chi@duke.edu
|
| Phone |
919-668-4759
|
| Organization name |
Duke University
|
| Department |
Molecular Genetics and Microbiology
|
| Street address |
101 Science Drive, DUMC 3382
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE61286 |
ACLY and ACC1 Regulate Hypoxia-Induced Apoptosis by Modulating ETV4 via α-ketoglutarate |
|
