|
| Status |
Public on May 11, 2016 |
| Title |
wilms tissue 2 |
| Sample type |
RNA |
| |
|
| Source name |
wilms tissue 61
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blastemal WT
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA including miRNAs was isolated using theQiagen miRNeasy Kit according to manufacturers recommendations. RNA concentration was measured using NanoDrop2000 (ThermoScientific). RNA integrity was measured using the PicoRNA kit (Agilent) on a Bioanalyzer2100.
|
| Label |
Cy3
|
| Label protocol |
The mRNA expression profile was determined using Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray according to the manufacturers recommendations
|
| |
|
| Hybridization protocol |
The mRNA expression profile was determined using Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray according to the manufacturers recommendations
|
| Scan protocol |
Array´s were scanned with the Agilent Scanner with default settings.
|
| Data processing |
Raw expression values were extracted using the Feature Extraction Software and GeneSpring Software of Agilent. Data was quantil normalized.
|
| |
|
| Submission date |
Mar 02, 2015 |
| Last update date |
Apr 23, 2018 |
| Contact name |
Nicole Ludwig |
| E-mail(s) |
n.ludwig@mx.uni-saarland.de
|
| Phone |
004968411626269
|
| Organization name |
Saarland University
|
| Department |
Human Genetics
|
| Street address |
Kirrberger Strasse
|
| City |
Homburg |
| ZIP/Postal code |
66421 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE66405 |
mRNA expression profiles in Wilms tumor subtypes |
|
| Relations |
| Reanalyzed by |
GSE113533 |
