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Sample GSM1613603 Query DataSets for GSM1613603
Status Public on Mar 01, 2015
Title C1 Large live cell 1
Sample type SRA
 
Source name Primary foreskin fibroblast cells
Organism Homo sapiens
Characteristics cell line: CRL2097
cell type: large live
Extracted molecule polyA RNA
Extraction protocol Cells were added to the Fluidigm C1 Single Cell Auto Prep System at 200K cells/ml. ERCC control mix 1 was added at a dilution of 1:10,000.
First- and second-strand synthesis were performed on the C1 Single Cell Auto Prep System. Final library prep was performed using the Illumina Nextera XT DNA sample preparation kit, using the modified protocol in the C1 manual.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description sample id: Live-PL2-A01
processed data file: singlecellseq_star_HTSeqCounts.tsv
Data processing Aligned reads to genome using STAR
Assigned counts per gene using HTSeq
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include counts for all genes and samples. All single-cell data is compiled into a single .tsv file, and there is a separate .txt file for the bulk data from each cell line.
 
Submission date Feb 18, 2015
Last update date May 15, 2019
Contact name Arjun Raj
Organization name University of Pennsylvania
Department Bioengineering
Street address 210 S. 33rd St., Ste. 240 Skirkanich Hall
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL18573
Series (1)
GSE66053 Single mammalian cells compensate for differences in cellular volume and DNA copy number through independent global transcriptional mechanisms
Relations
BioSample SAMN03353382
SRA SRX883407

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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