|
| Status |
Public on Feb 10, 2017 |
| Title |
Flk1+MPC |
| Sample type |
RNA |
| |
|
| Source name |
Flk1+ MPC as cardiovascular progenitors
|
| Organism |
Mus musculus |
| Characteristics |
cell type: embryonic stem cells
time point: day 4.5
|
| Treatment protocol |
CsAYTE was treated whenever medium was changed. DMSO was treated as a control vehicle.
|
| Growth protocol |
For induction and differentiation of Flk1+ MPC, sfPCB, PCB, and M+CM, mouse PSC were cultured in differentiation medium without leukemia inhibitory factor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Amplified and labeled cRNA was purified on cRNA Cleanup Module (Agilent Technology) according to the manufacturer’s protocol. Labeled cRNA target was quantified using ND-1000 spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE).
|
| |
|
| Hybridization protocol |
After checking labeling efficiency, fragmentation of cRNA was performed by adding 10X blocking agent and 25X fragmentation buffer and incubating at 60oC for 30 min. The fragmented cRNA was resuspended with 2X hybridization buffer and directly pipetted onto assembled Agilent’s Mouse Oligo Microarray (44K). The arrays hybridized at 65oC for 17 hours using Agilent Hybridization oven (Agilent Technology, USA). The hybridized microarrays were washed as the manufacturer’s washing protocol (Agilent Technology, USA).
|
| Scan protocol |
The hybridized images were scanned using Agilent’s DNA microarray scanner and quantified with Feature Extraction Software (Agilent Technology, Palo Alto, CA).
|
| Description |
Gene expression at day 4.5
|
| Data processing |
All data normalization and further analysis were performed using GeneSpringGX 7.3 (Agilent Technology, USA).
|
| |
|
| Submission date |
Feb 10, 2015 |
| Last update date |
Feb 10, 2017 |
| Contact name |
Gou Young Koh |
| E-mail(s) |
gykoh@kaist.ac.kr
|
| Phone |
82-42-350-2638
|
| Organization name |
KAIST
|
| Street address |
291 Daehark-ro, Yuseong-gu
|
| City |
Daejeon |
| ZIP/Postal code |
305-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE65791 |
Generation of PDGFRα+ Cardioblasts from Pluripotent Stem Cells |
|
