|
| Status |
Public on Feb 09, 2015 |
| Title |
R14del-CMs AAV6-Luc miRNA |
| Sample type |
SRA |
| |
|
| Source name |
Cardiomyocytes derived from PLN-R41del iPSC cells infected with AAV6-EGFP-miR-luc
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: iPS-derived cardiomyocytes
differentiation stage: Day 30 post-differentiation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cardiomyocytes derived from PLN-R41del iPSC cells were infected with AAV6-EGFP-miR-PLN or AAV6-EGFP-miR-luc as a negative control at an MOI of 1e4. One week after infection, total RNA was extracted using Zymo columns and 2 micrograms were used to generate an RNAseq sequencing library.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Read alignment with Star under default configurations
Gene raw read counts were determined with Htseq under default conditions
Genome_build: hg19
|
| |
|
| Submission date |
Feb 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
FRANCESCA STILLITANO |
| E-mail(s) |
francesca.stillitano@mssm.edu
|
| Phone |
+12128249020
|
| Organization name |
Mount Sinai School of Medicine
|
| Department |
CARDIOVASCULAR RESEARCH
|
| Street address |
1470 Madison Avenue
|
| City |
NEW YORK |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (2) |
| GSE65761 |
Correction of human phospholamban R14del mutation associated with cardiomyopathy using targeted nucleases and combination therapy [RNA-Seq] |
| GSE65763 |
Correction of human phospholamban R14del mutation associated with cardiomyopathy using targeted nucleases and combination therapy |
|
| Relations |
| BioSample |
SAMN03333465 |
| SRA |
SRX869318 |
