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Sample GSM1600330 Query DataSets for GSM1600330
Status Public on Feb 04, 2015
Title MCL001_FP-MERS_7hr_2_microRNA
Sample type RNA
 
Source name Calu-3 cells, FP-MERS treatment, 7 hours, replicate 2
Organism Homo sapiens
Characteristics time: 7 hours
virus: FP-MERS
replicate: 2
Treatment protocol Cells were washed twice with PBS and infected with a multiplicity of infection of 5.
Growth protocol SCL002.0P: Maintenance, Plating and Virus Infection of Calu-3 cells
Extracted molecule total RNA
Extraction protocol TCL001.0P: Preparation of Samples from Calu-3 cells for Isolation of RNA.
Label Cy3
Label protocol Total RNA from each sample was labeled using miRNA Complete Labeling and Hyb Kit (v2.4, Agilent Technologies)
 
Hybridization protocol Human miRNA MicroarraySlide (8x60K, Agilent Technologies)
Scan protocol Agilent Scanner G2505C
Data processing Scanned images were analyzed using Agilent Feature Extraction Software (v11.0.1.1). Quantile normalization and data processing performed using GeneSpring GX v12.0 software (Agilent Technologies).
 
Submission date Feb 03, 2015
Last update date Aug 31, 2016
Contact name Natalie Heller
E-mail(s) natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platform ID GPL19730
Series (2)
GSE65574 Human Calu-3 cell transcriptome response to a wild type infectious clone of Middle Eastern Respiratory Syndrome (icMERS) coronavirus and icMERS mutant viruses
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_25_P00010019 2.761805414
A_25_P00010020 2.70285931
A_25_P00010021 3.159522238
A_25_P00010023 2.952272784
A_25_P00010041 2.572632578
A_25_P00010042 2.897758628
A_25_P00010043 2.855127279
A_25_P00010044 2.416000155
A_25_P00010047 3.048639333
A_25_P00010048 3.107703868
A_25_P00010053 2.911237584
A_25_P00010054 2.850635378
A_25_P00010062 2.840717351
A_25_P00010063 3.011251077
A_25_P00010070 3.072441956
A_25_P00010071 3.0915541
A_25_P00010072 3.079178291
A_25_P00010073 3.09635796
A_25_P00010078 2.991686038
A_25_P00010079 2.832054845

Total number of rows: 4774

Table truncated, full table size 125 Kbytes.




Supplementary file Size Download File type/resource
GSM1600330_MCL001_FP-MERS_7hr_2_microRNA.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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