|
| Status |
Public on Dec 31, 2015 |
| Title |
SOC-20, differentiated |
| Sample type |
RNA |
| |
|
| Source name |
KT20-ad
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: differentiated ovarian cancer stem cells
|
| Treatment protocol |
Differentiation of spheroid cells were induced by cultivating dissociated spheroid cells on standard culture dishes in the presence of 10% fetal bovine serum for 14 days.
|
| Growth protocol |
Undifferentiated ovarian cancer stem cells were grown in grown in STEMPRO hESC SFM (Gibco) supplemented with 8 ng/ml bFGF (Invitrogen), penicillin/streptomycin, and in the presence or absence of 20 μM Y27632 (Wako) and/or 10 μg/ml insulin on ultra-low-attachment culture dishes (Corning).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using miRNeasy mini kit (QIAGEN) according to the manufacturer’s protocol. RNA was quantified using a NanoDrop-1000 spectrophotometer. RNA quality control was performed in 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA).
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA was Cy3-labeled according to the manufacturer's protocol of On-Color Microarray-Based Gene Expression Analyses (Low Input Quick Amp Labeling), ver 6.5.
|
| |
|
| Hybridization protocol |
0.6 ug of labelled cRNA was fd hybridized to Agilent Whole Human Genome Oligo Microarrays (G4851B, Agilent) for 17 hours at 65C in a rotating Agilent hybridization oven.
|
| Scan protocol |
Slides were scanned on the Agilent DNA Microarray Scanner (Agilent).
|
| Description |
Gene expression after differentiation
SAMPLE 8
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7.1.1(Agilent) using default parameters (protocol GE1_107_Sep09 and Grid: 039494_D_F/20120628).
|
| |
|
| Submission date |
Jan 15, 2015 |
| Last update date |
Apr 23, 2018 |
| Contact name |
koji okamoto |
| E-mail(s) |
kojokamo@ncc.go.jp
|
| Phone |
81335422511
|
| Organization name |
National Cancer Center Research Institute
|
| Department |
Division of Cancer Differentiation
|
| Street address |
5-1-1 Tsukiji
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
1040045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE64999 |
Gene regulation during differentiation of ovarian cancer stem cells |
|
| Relations |
| Reanalyzed by |
GSE113533 |
