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Sample GSM1575604 Query DataSets for GSM1575604
Status Public on Jan 02, 2015
Title 15 mins exposure to isofurane, biological rep 8
Sample type RNA
Source name Brain cortex from rat exposed to isoflurane
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
gender: male
age: 20 weeks
weight: 250-300g
sample group: 15 mins exposure to isofurane
tissue: brain cortex
Treatment protocol Rats were exposed to isoflurane in oxygen at a concentration of 2% (1.4 MAC) or pure oxygen for 15 minutes and allowed to recover for 6 hours. Brain was removed and cortex dissected and placed into RNA later (Invitrogen, Paisly, UK) for RNA preservation.
Growth protocol 20 week old Sprague Dawley rats between 250-300g
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIZOL (Invitrogen, Paisly, UK). Total RNA was further purified using Quiagen column based methodology (Quiagen Ltd, Manchester, UK)
Label biotin
Label protocol Biotinylated cRNA was prepared according to Affymetrix standard protocol using 10ug total RNA
Hybridization protocol Following fragmentation, 10ug of cRNA were hybridized for 16 hours @ 45oC to rat 230 2.0 genechips
Scan protocol Following hybridization and staining, gene chips were scanned using at 3mm resolution using a gene array scanner
Description 15_8
Data processing The data were analysed using Affymetrix microarray software suite 5.0 (MAS 5.0) using default analysis setting and global scaling as normalization method and Dchip ver 2010 using a baseline array for normalization and model based expression
affymetrix-algorithm-name = ExpressionStat
affymetrix-algorithm-version = 5.0
program-name = Expression Console
program-id =
Submission date Dec 31, 2014
Last update date Jan 02, 2015
Contact name Damon Lowes
Phone +441561362361
Organization name University of Aberdeen
Department Immunology, Infection and inflammation
Lab Anaesthesia and Intensive care
Street address IMS, Foresterhill
City Aberdeen
State/province Aberdeenshire
ZIP/Postal code AB252ZD
Country United Kingdom
Platform ID GPL1355
Series (1)
GSE64617 Gene expression and complex network analyis of effects of anaethesia

Data table header descriptions
VALUE ExpressionStat 5.0
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 469.702 P 0.000753643
AFFX-BioB-M_at 630.422 P 5.16732e-05
AFFX-BioB-3_at 415.15 P 4.42873e-05
AFFX-BioC-5_at 1300.23 P 9.4506e-05
AFFX-BioC-3_at 1544.09 P 4.42873e-05
AFFX-BioDn-5_at 2653.98 P 6.02111e-05
AFFX-BioDn-3_at 5023.32 P 8.14279e-05
AFFX-CreX-5_at 14872.1 P 5.16732e-05
AFFX-CreX-3_at 15640.2 P 4.42873e-05
AFFX-DapX-5_at 21.5368 A 0.123572
AFFX-DapX-M_at 20.8633 A 0.123572
AFFX-DapX-3_at 3.17542 A 0.804754
AFFX-LysX-5_at 17.0995 A 0.0956669
AFFX-LysX-M_at 2.27966 A 0.686277
AFFX-LysX-3_at 11.6848 A 0.227636
AFFX-PheX-5_at 2.70511 A 0.937071
AFFX-PheX-M_at 1.74814 A 0.932322
AFFX-PheX-3_at 33.1389 A 0.131361
AFFX-ThrX-5_at 16.0232 A 0.382599
AFFX-ThrX-M_at 3.79716 A 0.5

Total number of rows: 31099

Table truncated, full table size 953 Kbytes.

Supplementary file Size Download File type/resource
GSM1575604_15_8.CEL.gz 2.7 Mb (ftp)(http) CEL
GSM1575604_15_8.CHP.gz 257.9 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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