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Sample GSM1575598 Query DataSets for GSM1575598
Status Public on Jan 02, 2015
Title 15 mins exposure to isofurane, biological rep2
Sample type RNA
 
Source name Brain cortex from rat exposed to isoflurane
Organism Rattus norvegicus
Characteristics strain: Sprague Dawley
gender: male
age: 20 weeks
weight: 250-300g
sample group: 15 mins exposure to isofurane
tissue: brain cortex
Treatment protocol Rats were exposed to isoflurane in oxygen at a concentration of 2% (1.4 MAC) or pure oxygen for 15 minutes and allowed to recover for 6 hours. Brain was removed and cortex dissected and placed into RNA later (Invitrogen, Paisly, UK) for RNA preservation.
Growth protocol 20 week old Sprague Dawley rats between 250-300g
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIZOL (Invitrogen, Paisly, UK). Total RNA was further purified using Quiagen column based methodology (Quiagen Ltd, Manchester, UK)
Label biotin
Label protocol Biotinylated cRNA was prepared according to Affymetrix standard protocol using 10ug total RNA
 
Hybridization protocol Following fragmentation, 10ug of cRNA were hybridized for 16 hours @ 45oC to rat 230 2.0 genechips
Scan protocol Following hybridization and staining, gene chips were scanned using at 3mm resolution using a gene array scanner
Description 15_2
Data processing The data were analysed using Affymetrix microarray software suite 5.0 (MAS 5.0) using default analysis setting and global scaling as normalization method and Dchip ver 2010 using a baseline array for normalization and model based expression
affymetrix-algorithm-name = ExpressionStat
affymetrix-algorithm-version = 5.0
program-name = Expression Console
program-id = 1.4.0.38
 
Submission date Dec 31, 2014
Last update date Jan 02, 2015
Contact name Damon Lowes
E-mail(s) damon.lowes@outlook.com
Phone +441561362361
Organization name University of Aberdeen
Department Immunology, Infection and inflammation
Lab Anaesthesia and Intensive care
Street address IMS, Foresterhill
City Aberdeen
State/province Aberdeenshire
ZIP/Postal code AB252ZD
Country United Kingdom
 
Platform ID GPL1355
Series (1)
GSE64617 Gene expression and complex network analyis of effects of anaethesia

Data table header descriptions
ID_REF
VALUE ExpressionStat 5.0
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 540.871 P 0.000296708
AFFX-BioB-M_at 979.112 P 5.16732e-05
AFFX-BioB-3_at 602.655 P 4.42873e-05
AFFX-BioC-5_at 1751.73 P 9.4506e-05
AFFX-BioC-3_at 2020.4 P 5.16732e-05
AFFX-BioDn-5_at 3835.99 P 5.16732e-05
AFFX-BioDn-3_at 6682.02 P 7.00668e-05
AFFX-CreX-5_at 23058.8 P 5.16732e-05
AFFX-CreX-3_at 21813.5 P 4.42873e-05
AFFX-DapX-5_at 2.25996 A 0.804754
AFFX-DapX-M_at 8.10789 A 0.544587
AFFX-DapX-3_at 4.30518 A 0.916408
AFFX-LysX-5_at 10.3757 A 0.139482
AFFX-LysX-M_at 13.3948 A 0.340661
AFFX-LysX-3_at 14.7963 A 0.300606
AFFX-PheX-5_at 12.4451 A 0.60308
AFFX-PheX-M_at 1.82665 A 0.949771
AFFX-PheX-3_at 15.6721 A 0.559359
AFFX-ThrX-5_at 4.62538 A 0.574038
AFFX-ThrX-M_at 4.18968 A 0.5

Total number of rows: 31099

Table truncated, full table size 954 Kbytes.




Supplementary file Size Download File type/resource
GSM1575598_15_2.CEL.gz 2.6 Mb (ftp)(http) CEL
GSM1575598_15_2.CHP.gz 259.2 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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