|
| Status |
Public on Sep 08, 2015 |
| Title |
luminal_CD133_negative_3sort25 |
| Sample type |
RNA |
| |
|
| Source name |
mouse mammary tumor arising from transplanted epithelial cells
|
| Organism |
Mus musculus |
| Characteristics |
donor/recipient strain: FVB/NJ
source cell type: mouse mammary epithelial cells
transduced with: PyMT-expressing lentivirus
cell type: luminal_CD133_negative
cd133: negative
cd24: high
cd49f: low
lung metastasis: No
|
| Growth protocol |
Cells were transduced with PyMT-expressing lentivirus, sorted, and transplanted into syngeneic cleared mouse mammary fat pads. RNA was isolated from tumors grown from transplanted cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen RNEasy kit
|
| Label |
Cy3
|
| Label protocol |
Total RNA was labeled with Cy3 using the Agilent One-Color Quick Amp Labeling Protocol
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized to arrays using the standard Agilent 1-color gene expression hybridization and wash protocol
|
| Scan protocol |
Arrays were scanned on an Agilent G2505C Microarray Scanner at 5 micron resolution
|
| Description |
10822X2
|
| Data processing |
Images were processed using the Agilent Feature Extraction software version 10.5.1.1.
Control features and nonuniform array features were removed. Remaining features for each probe were averaged and log base 2 transformed.
Log2 intensities were quantile normalized.
|
| |
|
| Submission date |
Dec 22, 2014 |
| Last update date |
Sep 08, 2015 |
| Contact name |
Brett Milash |
| E-mail(s) |
brett.milash@utah.edu
|
| Organization name |
University of Utah
|
| Department |
Center for High Performance Computing
|
| Street address |
155 S. 1452 E. Room 414
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE64453 |
Transformation of enriched mammary cell populations with Polyoma Middle T Antigen influences tumor subtype and metastatic potential |
|
