|
| Status |
Public on Sep 23, 2019 |
| Title |
Control, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Leukemia cells (MLL-AF9) of control group
|
| Organism |
Mus musculus |
| Characteristics |
cell type: leukemia cells (MLL-AF9)
protocol: control mice
tissue: spleen
|
| Treatment protocol |
After sorting, cells were centrifuged at 1400rpm and Trizol were added into the tube
|
| Growth protocol |
leukemia cells were sorted by FACS from control groups and Dox inducible groups at different time points
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with TRIzol reagent (Invitrogen, Canada) and purified using RNeasy Mini Kit (Qiagen, German)
|
| Label |
biotin
|
| Label protocol |
RNA was labeled using the GeneChip® WT Terminal Labeling and Controls Kit combined with the Ambion® WT Expression Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, Labeled cRNA was hybridized to Mouse Gene 2.0 ST GeneChip® arrays (Affymetrix, America).
|
| Scan protocol |
GeneChips were scanned by the GeneChip® Scanner 3000 7G
|
| Description |
Gene expression data from control leukemia cells
|
| Data processing |
Raw data (CEL files) were normalized at transcript level using MAS5.0
|
| |
|
| Submission date |
Dec 22, 2014 |
| Last update date |
Sep 23, 2019 |
| Contact name |
Hui Cheng |
| E-mail(s) |
chenghui@ihcams.ac.cn
|
| Organization name |
Institute of Hematology
|
| Street address |
288 Nanjing road
|
| City |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE64414 |
Selective elimination of leukemia cells by reprogramming factors |
|
